Effect of hypoxia on equine mesenchymal stem cells derived from bone marrow and adipose tissue

BMC Veterinary Research

Table 4 Summary of gene information

Gene	Accession number	Primer sequence (5’-3’)	Amplicon size (bp)
CD29 ^a	XM_001492665	F: GTAAAAAGTCTTGGAACCGATCTGAT	81
		R: CACAAATGAGCCAAACCCAATT
CD34 ^a	XM_001491596	F:CACTAAACCCTCTACATCATTTTCTCCTA	150
		R: GGCAGATACCTTGAGTCAATTTCA
CD44 ^a	NM_001085435	F: CCCACGGATCTGAAACAAGTG	95
		R: TTCTGGAATTTGAGGTCTCCGTAT
CD49d ^a	XM_001917601	F: CATCGGCCTTCTCACAGAGAA	101
		R: GCCATTATTGTCTGCATCAATTTG
CD73 ^a	XM_001500115	F: GGGATTGTTGGATACACTTCAAAAG	90
		R: GCTGCAACGCAGTGATTTCA
CD90 ^a	EU881920	F: TGCGAACTCCGCCTCTCT	93
		R: GCTTATGCCCTCGCACTTG
CD105 ^a	XM_001500078	F: GACGGAAAATGTGGTCAGTAATGA	100
		R: GCGAGAGGCTCTCCGTGTT
CD106 ^a	NM_001101650	F: CATCGTGACCTGTGGGCATA	111
		R: TGGGTTTCCCTCCACTAGCA
CD146 ^a	XM_001917594	F: CTGGACTTGGAAACCACAACATC	85
		R: CAGGTCTCACTCGGACATCAGA
CD166 ^a	XM_001503380	F: GTCTGGTCTTCTGCCTCTTGATC	103
		R: TCGGCAAGGCATGATAATAGTG
HIF-1α	XM_001493206	F: AATCCAAAGATCCTGGCGTTG	103
		R: GCTGCTGTAGTAATGCGCCAAT
OCT4 ^b	XM_001490108	F: AGAGGCAACCTGGAGAACATG	70
		R: GGGCAATGTGGCTGATCTG
NANOG ^b	XM_001498808	F: TACCTCAGCCTCCAGCAGAT	119
		R: CAGTTGTTTTTCTGCCACCT
SOX2 ^c	FJ356148	F: TGGTTACCTCTTCCTCCCACT	178
		R: GGGCAGTGTGCCGTTAAT
GAPDH ^a	NM_001163856	F: GGCAAGTTCCATGGCACAGT	128
		R: CACAACATATTCAGCACCAGCAT
B2M ^a	NM_001082502	F: TCGTCCTGCTCGGGCTACT	102
		R: ATTCTCTGCTGGGTGACGTGA

^a previously described in [43].
^b previously described in [40].
^c previously described in [47].
Genes analysed, GenBank accession numbers, primer sequences for reverse transcriptase RT-PCR (F: forward and R: reverse) and amplicon sizes in base pairs (bp).

ISSN: 1746-6148