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Table 3 Comparison of real-time RT-PCR and RT-LAMP for the detection of BRV in clinical samples

From: Reverse transcription loop-mediated isothermal amplification assay for rapid detection of Bovine Rotavirus

Location of samples Number of Samples Number of positive samples for assay
Real-time RT-PCR RT-LAMP Sequencing
Nanning 24 7 7 correct
Liuzhou 15 3 3 correct
Fangcheng 10 3 3 correct
Shangsi 13 5 5 correct
Guilin 20 10 10 correct
Hengxian 16 1 1 correct
Total 88 29 29 correct
  1. a RT-LAMP -positive samples were all confirmed to be BRV by sequencing the RT-LAMP- products restriction analysis of VP6 genes.