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Table 2 Details of oligonucleotide primers used for RT-LAMP assay

From: Reverse transcription loop-mediated isothermal amplification assay for rapid detection of Bovine Rotavirus

Primer name

sequence(5’-3’)

Genome position

FIPa = F2 + F1c

GTTGTGATCTGTTCAACGTGAATGA-gaattc-GGGTTTTACATTTCATAAACCAA

F1c,510-534

F2,467-489

BIPb = B2 + B1c

GCTCATGATAACTTGATGGG- gatatc-GCACATGAGTAGTCGAATCC

B1c,537-556

B2,597-616

F3

TTGCAAAATAGAAGACAAAGAAC

444-466

B3

GTTGCGTATTAGCTGGCG

625-642

LoopF

CTGAATAAGGGAAAATGTTTGGTTT

483-507

loopB

GATGTGGCTCAATGCGGG

560-577

  1. Genome position according to the bovine rotavirus complete genome sequence (GenBank accession no. K02254.1).
  2. a FIP is composed of F2 and F1c, and they are linked by a sequences of EcoRIof -gaattc-.
  3. b BIP is composed of B2 and B1c, and they are linked by a sequences of EcoRV of -gatatc-.
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BMC Veterinary Research

ISSN: 1746-6148

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