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Table 1 Pathogens used and RT-LAMP assay results

From: Reverse transcription loop-mediated isothermal amplification assay for rapid detection of Bovine Rotavirus

   Source RT-LAMP result
Agarose gel electrophoresis Color change after adding dye
Bovine rotavirus (BRV)
 NCDV
genotype
G6P[1]
CVCC + +
 BRV014 G6P[5] CVCC + +
GX-BRV-1 G6P[11] GVRI + +
GX-BRV-2 G6P[5] GVRI + +
GX-BRV-3 G10P[11] GVRI + +
GX-BRV-4 G6P[11] GVRI + +
GX-BRV-5 G6P[11] GVRI + +
GX-BRV-6 G6P[5] GVRI + +
GX-BRV-7 G6P[5] GVRI + +
GX-BRV-8 G10P[11] GVRI + +
Other bovine pathogens    
 Bovine virus diarrhea (BVDV) CVCC - -
Mycobacterium bovis (MB) GVRI - -
  Classical swine fever virus (CSFV) CVCC - -
  Infective bovine rhinotracheitis virus (IBRV) CVCC - -
  Bovine Coronavirus (BCV) GVRI - -
Negative control    
 Rectal swab of normal bovine1 GVRI - -
 Rectal swab of normal bovine2 GVRI - -
 Rectal swab of normal bovine3 GVRI - -
 Nasal swab of normal bovine GVRI - -
 Blood of normal bovine GVRI - -
cells    
 cell −1 GVRI - -
 cell −2 GVRI - -
 cell −3 GVRI - -
 Cell-4 GVRI - -
 cell −5 GVRI - -
  1. CVCC = China Veterinary Culture Collection Center.
  2. GVRI = Guangxi Veterinary Research Institute.
  3. Positive = +; Negative = −.