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Table 7 Immunophenotyping of CD3+/CD4+ and CD3+/CD8+ lymphocytes from chickens following inoculation with various plasmid constructs in Trial 2

From: Co-administration of avian influenza virus H5 plasmid DNA with chicken IL-15 and IL-18 enhanced chickens immune responses

DNAs inoculated groups

Trial 2

Weeks post inoculation

CD3+/CD4+

CD3+/CD8+

Week 3

Week 6

Week 3

Week 6

Control

2.96 ± 0.52

7.25 ± 0.71

1.50 ± 0.28

3.58 ± 1.15

pDisplay

2.83 ± 0.34

7.42 ± 0.47

1.51 ± 0.66

3.28 ± 0.71

pDis/H5

6.56 ± 0.26*

8.86 ± 0.17*

2.52 ± 1.00

5.49 ± 1.15* a

pDis/H5 + pDis/IL-15

10.65 ± 0.59* a c

13.59 ± 1.15* a c

2.26 ± 0.38

3.53 ± 0.59

pDis/H5 + pDis/IL-18

8.42 ± 1.08* b

11.61 ± 0.61* b

2.12 ± 0.81

4.22 ± 1.21

  1. Each value represents the mean percentages of T cell sub-populations ± SD. The percentage of CD3+/CD4+ and CD3+/CD8+ T cells before inoculation are recorded as 2.64 ± 0.40 and 0.69 ± 0.24, respectively. Statistically significant differences (P < 0.05) are indicated by an asterisk (*), treated compared with untreated groups; a, pDis/H5 compared with pDis/H5 + pDis/IL-15; b, pDis/H5 compared with pDis/H5 + pDis/IL-18; c, pDis/H5 + pDis/IL-15 compared with pDis/H5 + pDis/IL-18.
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BMC Veterinary Research

ISSN: 1746-6148

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