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Table 6 Immunophenotyping of CD3+/CD4+ and CD3+/CD8+ lymphocytes from chickens following inoculation with various plasmid constructs in Trial 1

From: Co-administration of avian influenza virus H5 plasmid DNA with chicken IL-15 and IL-18 enhanced chickens immune responses

DNAs inoculated groups

Trial 1

Weeks post inoculation

CD3+/CD4+

CD3+/CD8+

Week 2

Week 5

Week 2

Week 5

Control

2.23 ± 0.60

1.55 ± 0.26

0.91 ± 0.30

1.79 ± 0.58

pDisplay

2.55 ± 0.36

1.76 ± 0.14

1.16 ± 0.46

2.00 ± 0.35

pDis/H5

4.53 ± 0.33 *

4.19 ± 0.52* b

3.16 ± 0.56* a b

2.97 ± 0.55* a b

pDis/H5 + pDis/IL-15

6.98 ± 0.73* a c

6.28 ± 0.60* a c

1.94 ± 0.46*

1.64 ± 0.44

pDis/H5 + pDis/IL-18

4.46 ± 0.34*

3.45 ± 0.26*

2.31 ± 0.84*

1.95 ± 0.52

  1. Each value represents the mean percentages of T cell sub-populations ± SD. The percentage of CD3+/CD4+ and CD3+/CD8+ T cells before inoculation are recorded as 2.92 ± 0.40 and 0.52 ± 0.24, respectively. Statistically significant differences (P < 0.05) are indicated by an asterisk (*), treated compared with untreated groups; a, pDis/H5 compared with pDis/H5 + pDis/IL-15; b, pDis/H5 compared with pDis/H5 + pDis/IL-18; c, pDis/H5 + pDis/IL-15 compared with pDis/H5 + pDis/IL-18.