Table 3 Gene name, product size, and forward and reverse primer sequences of GeXP assays of chicken spleen
Gene name | Fragment size | Left sequence without universalsc | Right sequence without universalsd |
|---|---|---|---|
IL-15 | 187 | AAGGTTTCCGAGGCTTGTACC | GTTTTCTGACTCTCCGGCCT |
IL-12β | 194 | TGAAGGAGTTCCCAGATGCT | AAGAACGTCTTGCTTGGCTC |
IL-2 | 201 | TAGTCACGAGGGGACCTTTG | GACGGCTTTCTTTGATGAGC |
IL-1β | 208 | TCTTTGGCTGTATTTCGGTAGC | TGCACTCCTGGGTCTCAGTT |
IL-18 | 215 | CAAAGTGTACACCATCCTGGAA | AAAGACGATTCCGCTTTCTTC |
IL-4 | 222 | CAAGTCAAAGCCGCACATC | GAGGATCCACCAGCTTCTGT |
IL-6 | 236 | TCAACATCGCCACCTACAAG | TAAAAGCAACGGGACGGTAA |
IL-10 | 243 | GCTTGTGGTTCGTCCAGATT | GAACAACCATTTTCCCATGC |
IL-8 | 250 | GAGCATCCGGATAGTGAATGA | TTGAGGGAGGTGCTGCTG |
IFN-γ | 257 | CAACGCAGAAGAAACAGTGC | CCCCATTGCAAATGTTGTATC |
TNFSF13B | 264 | AGCCACAGCATCTTCTTCGT | TCGAAGGAGAGCCACTCATC |
TGFβ | 271 | CAACCCCCAATGTCTCTGTT | ACCAGGAAACAAGCTTGACG |
GMCSF | 279 | CCGTTTCAGGAACCAGAGAG | GTCTGGCTGCTGGACATTTT |
COL6A2a | 287 | ATTTTCAATCCAGGGACGAT | CTCCTCTTCTCGCAGGTGAA |
KANb | 325 | ATCATCAGCATTGCATTCGATTCCTGTTTG | ATTCCGACTCGTCCAACATC |