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Table 2 List of primers used in amplification of mRNA transcript of AIV and chicken cytokine genes

From: Co-administration of avian influenza virus H5 plasmid DNA with chicken IL-15 and IL-18 enhanced chickens immune responses

Primer name Primer sequence Gene size (bp)
H5f 5’ TGG GAG GAT GGA GTT CTT CTG GAC 3’ H5 gene/175
H5r 5’ TGG AGT TTG ACA CTT GGT GTT GCA 3’  
IL-15f 5’ AAC TTG CTC CAT AGG TTT CCG A 3’ IL-15 gene/126
IL-15r 5’ AGA GTT TTG TGT TGG CTG TGC CAT 3’  
IL-18f 5’ TTC GAA ACG TCA ATA GCC AGT TGC 3’ IL-18 gene/161
IL18r 5’ GGG GTC GAC ATT GTC ATA TTC CTC TGC 3’  
ß-Actinf 5’ CAT CTA TGA AGG CTA CGC CCT 3’ ACTB gene/155
ß-Actinr 5’ GCT TCT CCT TGA TGT CAC GCA CAA 3’