Table 2 List of primers used in amplification of mRNA transcript of AIV and chicken cytokine genes
Primer name | Primer sequence | Gene size (bp) |
|---|---|---|
H5f | 5’ TGG GAG GAT GGA GTT CTT CTG GAC 3’ | H5 gene/175 |
H5r | 5’ TGG AGT TTG ACA CTT GGT GTT GCA 3’ |  |
IL-15f | 5’ AAC TTG CTC CAT AGG TTT CCG A 3’ | IL-15 gene/126 |
IL-15r | 5’ AGA GTT TTG TGT TGG CTG TGC CAT 3’ |  |
IL-18f | 5’ TTC GAA ACG TCA ATA GCC AGT TGC 3’ | IL-18 gene/161 |
IL18r | 5’ GGG GTC GAC ATT GTC ATA TTC CTC TGC 3’ |  |
ß-Actinf | 5’ CAT CTA TGA AGG CTA CGC CCT 3’ | ACTB gene/155 |
ß-Actinr | 5’ GCT TCT CCT TGA TGT CAC GCA CAA 3’ |  |