RT-PCR analysis of mRNA extracted from spleen and muscles from chickens vaccinated with pDis/H5, pDis/H5 + pDis/IL-15 and pDis/H5 + pDis/IL-18. The products were resolved in 1% (w/v) agarose gel in TAE. M: 100 bp DNA ladder (Promega, Madison WI) or VC 100 bp Plus DNA ladder (Vivantis, Malaysia) Lane 1: ACTB; Lane 2: H5 transcript in left muscle tissues; Lane 3: H5 transcript in right muscle tissues; Lane 4: H5 transcript in spleen. Lane 5: IL-15 or IL-18 transcript in left muscle tissues; Lane 6: IL-15 or IL-18 transcript in right muscle tissues; Lane 7: IL-15 or IL-18 transcript in spleen. In (A): RT-PCR analysis of mRNA. In (B): No specific band was detected when RNA from (A) was analyzed by PCR without the RT part.