PCR amplification of (a) H5 gene from pCR2.1 vector and (b) IL-15 and IL-18 genes from pgm2n.pk009.p17 and pgm2n.pk010.c11, respectively. The DNA band was observed in (a) 1% (w/v) agarose gel. M: 1 kb DNA ladder (Promega, Madison WI); Lane 1: H5 gene (1722 bp) and (b) 2% (w/v) agarose gel. M: 100 bp DNA ladder (Promega, Madison, WI). Lane 1: IL-15 gene (564 bp); Lane 2: IL-18 gene (486 bp).