Skip to main content

Table 1 PCR primers and conditions

From: Constitutive phosphorylation of the mTORC2/Akt/4E-BP1 pathway in newly derived canine hemangiosarcoma cell lines

Gene

Primer sequence

Annealing temperature (°C)

product size (bp)

CD31

For: 5'-GCACACAAGAGGCATGGTAAC-3'

63.0

211

 

Rev: 5'-GAATGGAGCACCACAGGTTT-3'

  

vWF

For: 5'-GCAATGTCTCCTCTGATGAAG-3'

63.0

221

 

Rev: 5'-GTACAAGACAACCCCCTGCT-3'

  

VEGF-A

For: 5'-CGTGCCCACTGAGGAGTT-3'

64.0

249, 231, and 177

 

Rev: 5'-AAATGCTTTCTCCGCTCTGA-3'

  

flt-1

For: 5'-ACCCTAAAGAAAGGCCAAGA-3'

63.0

156

 

Rev: 5'-CATCAGAGAAGGCAGGAGATG-3'

  

flk-1

For: 5'-GGAGCTCCAGAATGTGTCCT-3'

66.0

187

 

Rev: 5'-GGTGCATGAAACTTCAATGGT-3'

  

bFGF

For: 5'-CACTTCAAGGACCCCAAGAG-3'

61.0

234

 

Rev: 5'-GAAGCACTCGTCAGTAACACAT-3'

  

FGFR-1

For: 5'-GAAGTCGGATGCTACAGAGAAA-3'

65.0

162

 

Rev: 5'-CGTAAGTTGCCTTTGGAAGC-3'

  

HGF

For: 5'-ATGGGGAATGAGAAATGCAG-3'

60.0

210

 

Rev: 5'-AAAAATGCCAGGACGATTTG-3'

  

c-Met

For: 5-GATCTGGGCAGTGAATTAGT-3'

58.0

417

 

Rev: 5-GTCCAACAAAGTCCCATGAT-3'

  

IGF-I

For: 5'-AAGCAGCACTCATCCACGAT-3'

64.0

281

 

Rev: 5'-CAGCAGTCTTCCAACCCAAT-3'

  

IGF-IR

For: 5'-ACAACTACGCCCTGGTCATC-3'

64.0

295

 

Rev: 5'-CAGCGATTTGTAGTCCAGCA-3'

  

EGF

For: 5'-CTGTGGGATGCAGTACATGG-3'

61.0

204

 

Rev: 5'-CTCGGTAGCCTTCTGAGCAC-3'

  

EGF-R

For: 5'-AGGAGAGGAGAACTGCCAGA-3'

63.0

250

 

Rev: 5'-CAGGTGGCACCAAAGCTGTA-3'

  

PDGFB

For: 5'-TTGTACGGAAGAAGCCAACC-3'

64.0

279

 

Rev: 5'-CCTCAATCTCCTCCAGATGC-3'

  

PDGFR-α

For: 5'-GCCCCATTTACATCATCACC-3'

64.0

213

 

Rev: 5'-TGTCAGCTTGCTTCATGTCC-3'

  

PDGFR-β

For: 5'-ATGCAGTGCAGACTGTGGTC-3'

59.0

190

 

Rev: 5'-TCAGCACTAGGGATGTGCAG-3'

  

β-actin

For: 5'-ATTGAGCACGGCATCGTC-3'

65.5

261

 

Rev: 5'-GTCACCGGAGTCCATCACG-3'

  
Back to article page

BMC Veterinary Research

ISSN: 1746-6148

Contact us