Figure 4
Cell proliferation in the presence of growth factors. Typical results of cell proliferation assays after incubation with recombinant human VEGF. Each cell line was plated at 1 × 103 cells per well in 200 μL Medium 199 containing 10% FBS in 96-well plates for 24 h. After 24 h-serum starvation, 0, 1, 10, 50, or 100 ng/ml of recombinant growth factor in Medium 199 containing 1% FBS was added to the cells, or the medium was changed to Medium 199 containing 10% FBS for 72 h. The relative viable cell number was assessed by the WST-1 assay. *; p < 0.05 compared with cells that were not exposed to growth factor.