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Table 4 Archived ERAV and ERBV isolates tested with rRT-PCR assays and cRT-PCR assays

From: Development of one-step TaqMan® real-time reverse transcription-PCR and conventional reverse transcription-PCR assays for the detection of equine rhinitis A and B viruses

Sample

Source of TCF

rRT-PCR Assays

cRT-PCR Assays

  

ERAV

ERBV

ERAV

ERBV

  

ERAV

ERBV1

ERBV2

ERAV 5'-UTR

ERAV Poly

ERAV Poly 2

ERBV 5'-UTR

ERBV Poly 1

ERBV Poly 2

ERBV OUTER 1c

ERBV OUTER 2c

ERBV INNER 1c

ERBV INNER 2c

Serotype ERAV

ERAV

NVSLa

+

+

+

+

PERV, P4 2004 A

GERCb

+

+

+

+

Plowright,P4 2004 A

GERC

+

+

+

+

T3 isolate P10/2004 A

GERC

+

+

+

+

T10 isolate P9/2004 A

GERC

+

+

+

+

945 isolate P4/2004 A

GERC

+

+

+

+

ERV-1 (A) Plowright P4

GERC

+

+

+

+

ERV-1 (A) PERV P4

GERC

+

+

+

+

Amp 87-73-69-945

GERC

+

+

+

+

NS-T3

GERC

+

+

+

+

NS-T10

GERC

+

+

+

+

U-187

GERC

+

+

+

+

Serotype ERBV

ERBV

NVSL

+

+

+

+

+

+

+

+

+

Swiss isolate P6 2004 B

GERC

+

+

+

+

+

+

+

+

NS CW

GERCd

+

+

+

+

+

+

+

+

+

U-198V

GERC

+

+

+

+

+

+

+

+

NS-SD

GERC

+

+

+

+

+

+

+

+

Mare 189

GERC

+

+

+

+

+

+

+

+

51-12NVS

GERC

+

+

+

+

+

+

+

+

57-10NVS

GERC

+

+

+

+

+

+

+

+

57-11NVS

GERC

+

+

+

+

+

+

+

+

57-13NVS

GERC

+

+

+

+

+

+

+

+

58-13 NVS

GERCd

+

+

+

+

+

+

+

+

  1. a National Veterinary Services Laboratories, Ames, IA.
  2. b Maxwell H. Gluck Equine Research Center. All archived samples from GERC were obtained from the late Dr. William H. McCollum.
  3. c These assays were developed by Black et al. (2007).
  4. d These two TCF samples were also included in a study done by Black et al. (2005).