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Table 2 Primers and probes used in the rRT-PCR assay developed by Quinlivan et al . (2010)

From: Development of one-step TaqMan® real-time reverse transcription-PCR and conventional reverse transcription-PCR assays for the detection of equine rhinitis A and B viruses

rRT-PCR Assay Name

Target Genes (GenBank Accession Number)

Primer or Probe Namesa

Sequence 5' to 3' and Nucleotide Location

Length of Fragment (bp)

ERAV

5'-UTR of ERAV (NC_003982)

ERAV 468F

CCAGGTAACCGGACAGCG (468-485)

118

  

ERAV 569R

GGCAGCGCTACCACAGG (569-585)

 
  

ERAV 508b

FAMb-CATTGCTCTGGATGGTGT-MGBc (508-525)

 

ERBV

5'-UTR of ERBV (NC_003983)

ERBV 77F

TGATGCTTGGCTCTCAGAAA (77-96)

132

  

ERBV 189R

GCAAACGACCAACACATCAA (189-208)

 
  

ERBV 171b

FAMb-CTTCCAACTAAACCC-MGBc (171-185)

 
  1. aF = Forward primer. R = Reverse primer. Pr = Probe.
  2. bReporter dye (FAM; 6-carboxyfluorescein) labeled nucleotide.
  3. cNonfluorescent quencher dye (MGB™; minor groove binding) labeled nucleotide.
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BMC Veterinary Research

ISSN: 1746-6148

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