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Table 1 Primers and probes used in the rRT-PCR assays

From: Development of one-step TaqMan® real-time reverse transcription-PCR and conventional reverse transcription-PCR assays for the detection of equine rhinitis A and B viruses

rRT-PCR Assay Name

Target Genes (GenBank Accession Number)

Primer or Probe Namesa

Sequence 5' to 3' and Nucleotide Location

Length of Fragment (bp)

ERAV

5'-UTR of ERAV (L43052)

ERAV F

AGCGGCKdTGCTGGATTTTC (397-415)

60

  

ERAV R

CATYeTGYCAGCTTGGTGACA (438-457)

 
  

ERAV Pr

FAMb-CGGTGCCATTGCT-MGBc (417-429)

 

ERBV1

5'-UTR of ERBV1 (NC_003983)

ERBV1 F

CCCCTTfCCCTGAAGATTGCT (148-167)

61

  

ERBV1 R

GGCAAACGACCAACACATCA (190-209)

 
  

ERBV1 Pr

FAM-TTCTTCCAACTAAACCC-MGB (169-185)

 

ERBV2

5'-UTR of ERBV2 (NC_003077)

ERBV2 F

CCCCAACCCTTGAGATTGCT (148-167)

 
  1. aF = Forward primer. R = Reverse primer. Pr = Probe.
  2. bReporter dye (FAM; 6-carboxyfluorescein) labeled nucleotide.
  3. cNonfluorescent quencher dye (MGB™; minor groove binding) labeled nucleotide.
  4. dK represents G or T.
  5. eY represents C or T.
  6. f Nucleotide difference between the forward primers of ERBV1 and ERBV2 are in bold.