ORFV-infection interferes with COP-I mediated vesicular transport. (A) MHC I co-localizes with COP-I vesicles after ORFV infection. In infected cells (m.o.i. 0.5) MHC I was stained with mAb W6/32 (green) and anti-β-COP antibody (red). A representative result of three experiments at 10 hpi (panels A-F) and 24 hpi (panels G-L) is shown. Cell nuclei are stained blue with DAPI. A distinct co-localization (merge, yellow) of MHC I and β-COP was found in infected cells. Note that non-infected cells had to be photographed with longer exposure times as infected cells for the sake of better MHC-I/ß-COP visualization. (B) ORFV induced expression levels of β-COP. ß-COP (95 kDa) was detectable by Western blot analysis in infected cells (m.o.i. 1.0; lanes +) during 24 to 36 hpi. Detection of cellular ß-actin demonstrates comparable protein loading.