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Table 1 Literature data regarding the diagnostic specificity and sensitivity of ELISA tests used to detect antibodies against M. agalactiae

From: Comparative assessment of two commonly used commercial ELISA tests for the serological diagnosis of contagious agalactia of small ruminants caused by Mycoplasma agalactiae

Kit origin

Antigen type

Diagnostic specificity

Diagnostic sensitivity

Tested on

References

Anses Sophia Antipolis France (a)

Total antigen 12 strains mixture

94%

48%

1017 sheep sera in 52 flocks from the French Pyrénées-Atlantiques department

[8]

Intervet(CHEKit®)(a)

Total antigen PG2 reference strain

99%

72%

1017 sheep sera in 52 flocks from the French Pyrénées-Atlantiques department

[8]

  

99%

76%

30 goat sera collected from an infected herd and 97 uninfected sheep sera from New Zealand

[7]

  

76%

74%

223 CA infected and 120 CA free sheep from Italy

[6]

POURQUIER-ELISAM. agalactiae (b)

Fusion protein P48

99%

82%

1017 sheep sera in 52 flocks from the French Pyrénées-Atlantiques department

[8]

  

100%

56%

30 goat sera collected from an infected herd and 97 uninfected sheep sera from New Zealand

[7]

  

-

57%

223 CA infected and 120 CA free sheep from Italy

[6]

Italy (c)

Fusion proteins P80 and P55

97%

94%

223 CA infected and 120 CA free sheep from Italy

[6]

Brazil (c)

Total antigen Unknown strain

95%

7789%

86 sera from 44 bacteriologically positive and 42 bacteriologically negative Brazilian goats

[5]

  1. (a) Formerly used commercial kit, (b) Test currently marketed and included in the study.
  2. (c) Non commercial tests.
  3. CA: contagious agalactia.