Table 1 Primers used for semi-quantitative RT-PCR and RACE
From: Transcriptome analysis of head kidney in grass carp and discovery of immune-related genes
Primer | Sequence (5′ to 3′) | Application |
|---|---|---|
291-F1 | ATGTGGGTGATAGTTGGTTTACAAT | Expression study |
291-R1 | GTAATTTCAGAAGCACAGTTGAGAG | Expression study |
357-F1 | CTATCGCATGATTGCCTACTCAGACT | Expression study |
357-R1 | ACAACATTTTCCATCTCAATCTCAG | Expression study |
788-F1 | GGTCTTAACGGAGAGAAGTGCGA | Expression study |
788-R1 | GACTCTTCCGGCACGTAACT | Expression study |
153-F1 | CCAGCATCACAGTGTTCAGGCAG | Expression study |
153-R1 | AGTGTGTAGTTGTGTTCACCCTCC | Expression study |
β-actin-F | CAGATCATGTTTGAGACC | Expression study |
β-actin-R | ATTGCCAATGGTGATGAC | Expression study |
291-F2 | CTCTCAACTGTGCTTCTGAAATTAC | 3’ RACE PCR |
291-R2 | ATTGTAAACCAACTATCACCCACAT | 5’ RACE PCR |
357-F2 | GGTATGATTATGACTAAAGCAGGAC | 3’ RACE PCR |
357-R2 | GTCCTGCTTTAGTCATAATCATACC | 5’ RACE PCR |
788-F2 | AGTTACGTGCCGGAAGAGTC | 3’ RACE PCR |
788-R2 | TCGCACTTCTCTCCGTTAAGAC | 5’ RACE PCR |
153-F2 | GGAGGGTGAACACAACTACACACT | 3’ RACE PCR |
153-R2 | CTGCCTGAACACTGTGATGCTGG | 5’ RACE PCR |