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Table 2 Oligonucleotide primers and restriction enzymes

From: Generation of recombinant Orfvirus using an enhanced green fluorescent protein reporter gene as a selectable marker

Primer Sequence Restriction enzyme*
GFPFw1 5'-AACTTAGAATTCGCCACCATGGTGAGCAAGGGCGA-3' EcoR I
GFPRv1 5'-ATCAATGCGGCCGCTTACTTGTACAGCTCGTCCA-3' Not I
GFPintrFw2 5'-GACGTAAACGGCCACAAGTT-3'  
GFPintrRv2 5'-ACTGGGTGCTCAGGTAGTGG-3'  
GFPseqFw3 5'-CGACCACTACCAGCAGAACA-3'  
GFPseqRv3 5'-AAGTCGTGCTGCTTCATGTG-3'  
113LFFw1 5'-AGGCCTCTAAGCTTCAGGTTCCGGCTTCAGATGCGCGT-3' Hind III
113LFRv1 5'-ATTCGCGTCGACCACCAACACTTCCATTGTTGCGGC-3' Sal I
113RFFw2 5'-TCTTATGCGGCCGCGAGCCGCCGATGCAGATCGAGGTA-3' Not I
113RFRv2 5'-ATTCGCAGATCTTCGATCGCCAGTGCGCGGCGCATG-3' Bgl II
113intrFw3 5'-CGCCGTAATATGCTTAACCGGAGC-3'  
113intrRv3 5'-CGGACCGTGTTGGTCGTTGGGTCT-3'  
113seqFw4 5'-TTAGCTTCCTTGTTTTTATC-3'  
113seqRv4 5'-GTCCTTCGGGTCAGAGTCC-3'  
116LFFw1 5'-GCCTCTACTAGTAGGAAGTGGCCTCGCCGACCACGA-3' Spe I
116LFRv1 5'-ATTCGCGTCGACGTGGATGTCTCTAAGGTTCAATAC-3' Sal I
116RFFw2 5'-TCTTATGCGGCCGCCTACCACTGGTACCAGCACCTCCT-3' Not I
116RFRv2 5'-ATTCGCAGATCTGGCGCTACAGGCGTCCTGCAGGAA-3' Bgl II
116intrFw3 5'-GAACAACACGTCAACCGATG-3'  
116intrRv3 5'-AGGTGTGGGTTGACTTCCAG-3'  
116seqFw4 5'-GTCGAGCAGATGTTCATGGA-3'  
116seqRv4 5'-ATGCTGCACTTCCTGGAGAT-3'  
001intrFw1 5'-CTCGGTGACCTGCCTGAC-3'  
001intrRv1 5'-CTCGCGCACGTCGTAGAT-3'  
  1. * Restriction enzyme sites are underlined in prime sequences
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