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Table 2 Oligonucleotide primers and restriction enzymes

From: Generation of recombinant Orfvirus using an enhanced green fluorescent protein reporter gene as a selectable marker

Primer

Sequence

Restriction enzyme*

GFPFw1

5'-AACTTAGAATTCGCCACCATGGTGAGCAAGGGCGA-3'

EcoR I

GFPRv1

5'-ATCAATGCGGCCGCTTACTTGTACAGCTCGTCCA-3'

Not I

GFPintrFw2

5'-GACGTAAACGGCCACAAGTT-3'

 

GFPintrRv2

5'-ACTGGGTGCTCAGGTAGTGG-3'

 

GFPseqFw3

5'-CGACCACTACCAGCAGAACA-3'

 

GFPseqRv3

5'-AAGTCGTGCTGCTTCATGTG-3'

 

113LFFw1

5'-AGGCCTCTAAGCTTCAGGTTCCGGCTTCAGATGCGCGT-3'

Hind III

113LFRv1

5'-ATTCGCGTCGACCACCAACACTTCCATTGTTGCGGC-3'

Sal I

113RFFw2

5'-TCTTATGCGGCCGCGAGCCGCCGATGCAGATCGAGGTA-3'

Not I

113RFRv2

5'-ATTCGCAGATCTTCGATCGCCAGTGCGCGGCGCATG-3'

Bgl II

113intrFw3

5'-CGCCGTAATATGCTTAACCGGAGC-3'

 

113intrRv3

5'-CGGACCGTGTTGGTCGTTGGGTCT-3'

 

113seqFw4

5'-TTAGCTTCCTTGTTTTTATC-3'

 

113seqRv4

5'-GTCCTTCGGGTCAGAGTCC-3'

 

116LFFw1

5'-GCCTCTACTAGTAGGAAGTGGCCTCGCCGACCACGA-3'

Spe I

116LFRv1

5'-ATTCGCGTCGACGTGGATGTCTCTAAGGTTCAATAC-3'

Sal I

116RFFw2

5'-TCTTATGCGGCCGCCTACCACTGGTACCAGCACCTCCT-3'

Not I

116RFRv2

5'-ATTCGCAGATCTGGCGCTACAGGCGTCCTGCAGGAA-3'

Bgl II

116intrFw3

5'-GAACAACACGTCAACCGATG-3'

 

116intrRv3

5'-AGGTGTGGGTTGACTTCCAG-3'

 

116seqFw4

5'-GTCGAGCAGATGTTCATGGA-3'

 

116seqRv4

5'-ATGCTGCACTTCCTGGAGAT-3'

 

001intrFw1

5'-CTCGGTGACCTGCCTGAC-3'

 

001intrRv1

5'-CTCGCGCACGTCGTAGAT-3'

 
  1. * Restriction enzyme sites are underlined in prime sequences