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Table 1 Comparison of generating rORFVs using the pSPV-EGFP and pZIPPY-neo/gus vectors

From: Generation of recombinant Orfvirus using an enhanced green fluorescent protein reporter gene as a selectable marker

OV gene

Rounds of plaque assay

Time to obtain the puried rOVs (days)

 

pSPV-EGFP

pZIPPY-neo/gus

pSPV-EGFP

pZIPPY-neo/gus

ORFV002

-a

16

-

80[23]b

ORFV012

-

20

-

>100C

ORFV024

-

19

-

95[24]

ORFV113

4

20

12

>100C

ORFV116

5

20

14

>100C

ORFV120

-

17

-

85

ORFV121

-

18

-

90[25]

  1. a Not tested; b Reference; c After 20 rounds of plaque assay, still contaminated with wild type virus.