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Table 1 Comparison of generating rORFVs using the pSPV-EGFP and pZIPPY-neo/gus vectors

From: Generation of recombinant Orfvirus using an enhanced green fluorescent protein reporter gene as a selectable marker

OV gene Rounds of plaque assay Time to obtain the puried rOVs (days)
  pSPV-EGFP pZIPPY-neo/gus pSPV-EGFP pZIPPY-neo/gus
ORFV002 -a 16 - 80[23]b
ORFV012 - 20 - >100C
ORFV024 - 19 - 95[24]
ORFV113 4 20 12 >100C
ORFV116 5 20 14 >100C
ORFV120 - 17 - 85
ORFV121 - 18 - 90[25]
  1. a Not tested; b Reference; c After 20 rounds of plaque assay, still contaminated with wild type virus.