Skip to main content

Table 1 Sheep transfused with different blood components from scrapie infected sheep.

From: Classical scrapie prions in ovine blood are associated with B lymphocytes and platelet-rich plasma

Transfusion types Transfusion outcomes
Blood component blood volume (mL)1 Donor ID Clinical status Donor PRNP Recipient PRNP Recipient ID Rectal tissues PrPSc detection by IHC dpt to first positive biopsy dpt at necropsy
Whole blood 50 3806/3807 Preclinical MVRQ/MVRQ MARQ/MVRQ 4128/4132/4134 Positive 156-235 267
  131 3178 Preclinical MARQ/MARQ MARQ/MARQ 3668 Positive 252 261
      MARQ/TARQ 3669/3670 Not detected   236-261
  135 3187 Clinical MARQ/MVRQ TARQ/MVRQ 3613/3622 Positive   185-213
   3188 Clinical MARQ/MVRQ TARQ/MVRQ 3628 Not detected   213
      MARQ/MVRQ 3672 Positive 123 215
  119-131 3199 Clinical MARQ/MARQ MARQ/MARQ 3665/3666/3667 Positive 222-252 236-261
Buffy coat 100 3345 Clinical MARQ/MARQ MARQ/MARQ 3823/3833 Positive 239-288 275-306
      MARQ/MARQ 3832 Positive 549 1259
      MARQ/TARQ 3819 Positive 757 1391
      MARQ/TARQ 3825 Not detected   1391
PBMCs 50 4073 Preclinical MVRQ/MVRQ MVRQ/MVRQ 4302 Positive 182 646
      MARQ/MVRQ 4288/4309 Positive 182 646
      MARQ/MVRQ 4304/4305 Positive   251
  100 3806/3807 Preclinical MVRQ/MVRQ MARQ/MVRQ 4118/4119/4123 Positive 153-214 306
      TARQ/MVRQ 4120 Positive 153 306
  116 3774 Preclinical MVRQ/MVRQ MVRQ/MVRQ 3824 Positive 175 183
      MARQ/MVRQ 3921 Positive 175 183
      MARQ/MVRQ 3920 Positive   187
  50@ 4125 Preclinical MVRQ/MVRQ MVRQ/MVRQ 4378/4380 Positive 125 181
      MVRQ/MVRQ 4379 Positive   181
CD72+ B cells 50# 4125 Preclinical MVRQ/MVRQ MVRQ/MVRQ 4384/4385/4386 Positive 125-152 181
CD21+ B cells 50& 4125 Preclinical MVRQ/MVRQ MVRQ/MVRQ 4381/4383 Positive 125-152 187
      MVRQ/MVRQ 4382 Positive   187
Platelet-rich 180 3199 Clinical MARQ/MARQ MARQ/MARQ 3606 Positive 218 357
plasma 100 3345 Clinical MARQ/MARQ MARQ/MARQ 3820 Positive 288 554
      MARQ/MARQ 3821 Not detected   552
Platelet-poor plasma 100 3345 Clinical MARQ/MARQ MARQ/MARQ 3822/3836/3838 Not detected   549
  1. abbreviations: PBMCs = peripheral blood mononuclear cells; IHC = immunohistochemistry on antemortem and postmortem rectal tissues; PRNP = haplotypes shown include deduced amino acids at codons 112, 136, 154, 171; dpt = days post-transfusion; cells labeled with magnetic beads: @CD18-labeled PBMCs; #CD72-labeled B cells (Pan B lymphocytes); &CD21-labeled CD21+ B cells (a subpopulation of B lymphocytes), these cells were labeled with primary and magnetic microbeads coupled secondary antibodies
  2. 1Total whole blood volume from which each inoculum was derived per recipient animal.
\