Table 1 Sheep transfused with different blood components from scrapie infected sheep.
From: Classical scrapie prions in ovine blood are associated with B lymphocytes and platelet-rich plasma
Transfusion types | Transfusion outcomes | ||||||||
|---|---|---|---|---|---|---|---|---|---|
Blood component | blood volume (mL)1 | Donor ID | Clinical status | Donor PRNP | Recipient PRNP | Recipient ID | Rectal tissues PrPSc detection by IHC | dpt to first positive biopsy | dpt at necropsy |
Whole blood | 50 | 3806/3807 | Preclinical | MVRQ/MVRQ | MARQ/MVRQ | 4128/4132/4134 | Positive | 156-235 | 267 |
| Â | 131 | 3178 | Preclinical | MARQ/MARQ | MARQ/MARQ | 3668 | Positive | 252 | 261 |
| Â | Â | Â | Â | Â | MARQ/TARQ | 3669/3670 | Not detected | Â | 236-261 |
| Â | 135 | 3187 | Clinical | MARQ/MVRQ | TARQ/MVRQ | 3613/3622 | Positive | Â | 185-213 |
| Â | Â | 3188 | Clinical | MARQ/MVRQ | TARQ/MVRQ | 3628 | Not detected | Â | 213 |
| Â | Â | Â | Â | Â | MARQ/MVRQ | 3672 | Positive | 123 | 215 |
| Â | 119-131 | 3199 | Clinical | MARQ/MARQ | MARQ/MARQ | 3665/3666/3667 | Positive | 222-252 | 236-261 |
Buffy coat | 100 | 3345 | Clinical | MARQ/MARQ | MARQ/MARQ | 3823/3833 | Positive | 239-288 | 275-306 |
| Â | Â | Â | Â | Â | MARQ/MARQ | 3832 | Positive | 549 | 1259 |
| Â | Â | Â | Â | Â | MARQ/TARQ | 3819 | Positive | 757 | 1391 |
| Â | Â | Â | Â | Â | MARQ/TARQ | 3825 | Not detected | Â | 1391 |
PBMCs | 50 | 4073 | Preclinical | MVRQ/MVRQ | MVRQ/MVRQ | 4302 | Positive | 182 | 646 |
| Â | Â | Â | Â | Â | MARQ/MVRQ | 4288/4309 | Positive | 182 | 646 |
| Â | Â | Â | Â | Â | MARQ/MVRQ | 4304/4305 | Positive | Â | 251 |
| Â | 100 | 3806/3807 | Preclinical | MVRQ/MVRQ | MARQ/MVRQ | 4118/4119/4123 | Positive | 153-214 | 306 |
| Â | Â | Â | Â | Â | TARQ/MVRQ | 4120 | Positive | 153 | 306 |
| Â | 116 | 3774 | Preclinical | MVRQ/MVRQ | MVRQ/MVRQ | 3824 | Positive | 175 | 183 |
| Â | Â | Â | Â | Â | MARQ/MVRQ | 3921 | Positive | 175 | 183 |
| Â | Â | Â | Â | Â | MARQ/MVRQ | 3920 | Positive | Â | 187 |
| Â | 50@ | 4125 | Preclinical | MVRQ/MVRQ | MVRQ/MVRQ | 4378/4380 | Positive | 125 | 181 |
| Â | Â | Â | Â | Â | MVRQ/MVRQ | 4379 | Positive | Â | 181 |
CD72+ B cells | 50# | 4125 | Preclinical | MVRQ/MVRQ | MVRQ/MVRQ | 4384/4385/4386 | Positive | 125-152 | 181 |
CD21+ B cells | 50& | 4125 | Preclinical | MVRQ/MVRQ | MVRQ/MVRQ | 4381/4383 | Positive | 125-152 | 187 |
| Â | Â | Â | Â | Â | MVRQ/MVRQ | 4382 | Positive | Â | 187 |
Platelet-rich | 180 | 3199 | Clinical | MARQ/MARQ | MARQ/MARQ | 3606 | Positive | 218 | 357 |
plasma | 100 | 3345 | Clinical | MARQ/MARQ | MARQ/MARQ | 3820 | Positive | 288 | 554 |
| Â | Â | Â | Â | Â | MARQ/MARQ | 3821 | Not detected | Â | 552 |
Platelet-poor plasma | 100 | 3345 | Clinical | MARQ/MARQ | MARQ/MARQ | 3822/3836/3838 | Not detected | Â | 549 |