Skip to main content
Figure 2 | BMC Veterinary Research

Figure 2

From: Sparse PrPScaccumulation in the placentas of goats with naturally acquired scrapie

Figure 2

Immunolabeling of shed and intact ovine and caprine placental tissue. Single immunolabeling of PrPSc (red) in placentomes of scrapie-infected sheep (a) and goat (d) using mAb F99/97.6.1 and AEC immunolabeling, showing the reduction in PrPSc accumulation in caprine cotyledons relative to most ovine cotyledons. Sections from the same blocks labeled with an isotype control mAb show no labelling (b and e). Double immunolabeling using anti-prion antibody L42 (fuscia) and anti-pan cytokeratin, a marker of fetal trophoblasts (brown), demonstrates that PrPSc distribution in fetal trophoblasts (long arrows) and adjacent multinucleated cells (short arrows) was similar in the ovine (c) and caprine (f) placentomes. Photomicrographs were taken with an Olympus BX40 microscope coupled with an Olympus Q-Color3 camera. Axiovision software was used for scaling and Photoshop for formatting. Bar = 50 μm.

Back to article page