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Figure 1 | BMC Veterinary Research

Figure 1

From: Sparse PrPScaccumulation in the placentas of goats with naturally acquired scrapie

Figure 1

Western blot analysis of brain and shed placental cotyledons from sheep and goats with naturally acquired scrapie. Western blot detection of PrPSc using monoclonal antibodies F99/97.6.1 and P4 showing proteinase K resistant bands in samples of brain (lanes 1 and 3) and shed placental cotyledons (lanes 2 and 4) from goats (lanes 1 and 2) and sheep (lanes 3 and 4) with naturally acquired scrapie. Brain and placenta from a scrapie free goat (lanes 5 and 6) show no banding. Loading volumes were adjusted to produce similar banding intensity for comparison of migration patterns: positive and negative goat brain 3.2 mg per lane, positive sheep brain 0.8 mg, positive and negative goat placenta 150 mg, sheep placenta 30 mg. Arrows indicate molecular weight markers migrating at 30 and 20 kDa.

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