Loop-mediated isothermal amplification (LAMP) assays for the species-specific detection of Eimeria that infect chickens

Table 2 LAMP diagnosis of eimerian infection from field samples compared with lesion scoring and multiplex PCR.

Pen no.	Lesion score¹ (species)				PCR²			LAMP
	Duodenum	Mid-intestine	Caecae	Duodenum	Mid-intestine	Caecae	Duodenum	Mid-intestine	Caecae
1	-	-	-	-	-	-	-	-	-
2	1 (Eac)	-	-	Eac	-	-	Eac	-	-
3	-	-	-	-	-	-	-	-	-
4	-	-	-	-	-	-	-	-	-
5	-	-	-	-	-	-	-	Ema	-
6	-	-	-	-	-	-	-	-	-
7	1 (Eac)	-	-	Eac	-	-	Eac	-	-
8	-	-	-	-	-	-	-	-	-
9	1 (Eac)	2 (Ema)	-	Eac	Ema	-	Eac	Ema	-
10	-	1 (Ema)	-	-	Ema	-	-	Ema	-

Underlined entries were only detected using LAMP.
¹Lesion scoring performed as described by Johnson and Reid [8]. ²Multiplex PCR performed as described by Fernandez et al [30].

ISSN: 1746-6148