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Table 1 Oligonucleotides developed for Eimeria species-specific LAMP PCR.

From: Loop-mediated isothermal amplification (LAMP) assays for the species-specific detection of Eimeria that infect chickens

Species

Target

Acc. no.

Primer ID

Sequence (5' - 3')

E. acervulina

Ac-AD18-953a

AY571534

Eac_F3

CCTAACATTTCGCTTCACGGAC

   

Eac_B3

ATGAGCAAGTGGAACACCTTG

   

Eac_FIP

AGAGCACAGTGGCAGTGC-AGCAGACAGCATGGCTTACCT

   

Eac_BIP

GAAGACCCTCTGAAGAACGGA-CCTTCTCACCGCTTACCGG

   

Eac_LB

TAAGGTTACACCCGTGGAGG

   

Eac_LF

GCCATGCACAAAGCGACTT

E. brunetti

Br-J18-626a

AY571556

Ebr_F3

GGCCATCAAGTTCCATGAGC

   

Ebr_B3

TCAACCTCCTGAGTGTGGTT

   

Ebr_FIP

GAAAATGCCTTCGTAGCTGCT-GCTGGGTACGGAGCGTCTT

   

Ebr_BIP

TACTTCCTAGGATCCATCCTCGC-AGTTTCGCTGCCGCCTC

   

Ebr_LB

GAAACGCTCGAACATGGC

   

Ebr_LF

CTTCTCCACAGACCCAGAGGT

E. maxima

EmMIC1

M99058

Ema_F3

ACTACGGAAAAGTGCGTAGCT

   

Ema_B3

CCTTCCTCCCTTCTGAAAACTG

   

Ema_FIP

GAGTCACTGCTGATGTACCAAAAG-GAACTATGCCGCTTTCCCCTG

   

Ema_BIP

AGAATGCGGATTTGTTAGCAGC-AGCAAGTCCAAGGTGTGTGTA

   

Ema_LB

CAAGCCTACGCGGACATC

   

Ema_LF

TTATGCAGCTGGGTCAACG

E. mitis

Mt-A09-716a

AY571506

Emi_F3

ACGATAGCCAAGACACGTAAGG

   

Emi_B3

CCCCGTGATAAGAGTAGGAACA

   

Emi_FIP

CGCGGGTCGTGAGATTTAAATTAT-GGAAGATCAGGACGGGCACT

   

Emi_BIP

GTTTCAGTTGATGAACAAGCGAGA-TGCGCCTCTAGAATCAAGACG

   

Emi_LB

TCCATGCATCCCCTTGTT

   

Emi_LF

CGTGGGCACAGATTGATTC

E. necatrix

Nc-AD10-702a

AY571565

Ene_F3

TGGCTTTCCCGCGTACC

   

Ene_B3

CGGCCCAACACAAAGACTG

   

Ene_FIP

CGCTTGAGTTTTAAGCTATGCACA-GACCCAAGCAGCTCACCAA

   

Ene_BIP

CGCCATGCCATTCAATGAACG-GAGGCATACCGGCGTTGTC

   

Ene_LB

GTCTGTAACTTGGGACGTTGT

   

Ene_LF

GAACAGCCGGAGCCTCTC

E. praecox

Pr-A09-1108a

AY571603

Epr_F3

GCCCTTGTATGTTGCTGTTTCT

   

Epr_B3

GCGCACGAATCTGAATCACAC

   

Epr_FIP

ATCTCCTCAAAGACTTTCGCGTA-GCGCTTGGCTATATCCATAGG

   

Epr_BIP

GCTCTCGTGGCATACTTGC-GCCAGGAGCCACTGATTGT

   

Epr_LB

GAATAGCATTGCCAGGTGG

   

Epr_LF

GTCCACTGTCATTAATATTGCTGC

E. tenella

Tn-E03-1161a

AY571629

Ete_F3

GCTTGTGAAGGTCAGCGTG

   

Ete_B3

GCTGAGTCCATACGTACTTCCT

   

Ete_FIP

GCCACTGCTATGGAAAGTCACAC-CATAACTGGCATGCAGGGGT

   

Ete_BIP

GTTTGGCCCGAAAGTTGTGAAGA-CGTCAGAAATTGCTGCCCAAT

   

Ete_LB

CGCATGTGCAGTTGAAGACA

   

Ete_LF

CCAAATGTATCTGCTAGTTATATTAACAAG

  1. aEimeria species-specific SCAR markers [30].