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Figure 3 | BMC Veterinary Research

Figure 3

From: Expression patterns of intestinal calcium transport factors and ex-vivo absorption of calcium in horses

Figure 3

Western blot analyses. Expression of VDR and CB proteins in the horse intestine was determined by western blot analysis. Tissue samples of the duodenum (DD), jejunum (JE), ileum (IL), cecum (CC), colon ascendens ventrale (CAV), colon ascendens dorsale (CAD) and colon descendens (CD) were used. The loading was controlled by ß-actin. A) A representative western blot of VDR protein expression is shown. In all of the animals, major bands of approximately 40 kDa were found in the DD, while minor bands were detectable in the jejunum and colon in three animals. B) Relative expression of VDR to ß-actin was determined by densitometry using ImageJ software. The highest expression was measured in the DD. C) The strongest bands of CB protein were detected in the duodenum in all of the animals. Minor bands were visible in the jejunum. In 9 animals, weak signals were found in cecum, and in 6 animals, weak bands were seen in the ileum and colon ascendens ventrale. D) The expression of CB protein relative to ß-actin was determined by measuring the optical density using ImageJ software. The highest CB protein expression was found in the DD.

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