Figure 1

Reverse transcriptase PCR. Detection of VDR, calbindin-D9k (CB), TRPV6 and TRPV5 cDNA in different intestinal segments (DD = duodenum, JE = jejunum, IL = ileum, CC = cecum, CAV = colon ascendens ventrale, CAD = colon ascendens dorsale, CD = colon descendens) detected by reverse transcriptase PCR. GAPDH was used as a loading control. VDR, TRPV6 and CB cDNA was found in all investigated segments (Figure 1A, B, C), while TRPV5 was solely detected in the kidney (KI), not in the horse intestine (Figure 1D).