|
Spot I.D.
|
Trmt*Day
|
LSM ± SEM
|
p-vlaue
|
Direction
|
|---|
|
11
|
P*14
|
0.03 ± 0.01
|
0.0205
|
↑
|
| |
Heba*14
|
0.02 ± 0.01
|
0.0382
|
↑
|
|
12
|
P*2
|
0.05 ± 0.02
|
0.0127
|
↑
|
|
13
|
P*2
|
0.02 ± 0.01
|
0.0206
|
↑
|
| |
Heba*14
|
0.02 ± 0.01
|
0.024
|
↑
|
|
21
|
P*14
|
0.03 ± 0.01
|
0.0397
|
↑
|
|
33
|
P*2
|
0.05 ± 0.02
|
0.0056
|
↑
|
|
35
|
NB*2
|
0.02 ± 0.004
|
0.0041
|
↑
|
| |
Heba*14
|
0.01 ± 0.005
|
0.0467
|
↑
|
|
39 (CPP3)
|
P*2
|
0.02 ± 0.007
|
0.0476
|
↑
|
| |
P*14
|
0.01 ± 0.004
|
0.0032
|
↑
|
| |
Heba*14
|
0.01 ± 0.004
|
0.0154
|
↑
|
|
45
|
NB*2
|
0.01 ± 0.004
|
0.0122
|
↑
|
|
131
|
NB*2
|
0.01 ± 0.005
|
0.031
|
↑
|
|
141
|
NB*2
|
0.04 ± 0.01
|
0.0296
|
↑
|
|
212
|
P*2
|
0.01 ± 0.004
|
0.0038
|
↑
|
| |
Heba*14
|
0.01 ± 0.005
|
0.0408
|
↑
|
|
220
|
P*2
|
0.04 ± 0.01
|
0.0214
|
↑
|
| |
Heba*14
|
0.01 ± 0.005
|
0.0304
|
↑
|
|
222
|
NB*2
|
0.02 ± 0.009
|
0.0384
|
↑
|
|
227
|
P*2
|
0.008 ± 0.003
|
0.0127
|
↑
|
|
235 (CPP3)
|
P*2
|
0.03 ± 0.01
|
0.0241
|
↑
|
| |
P*14
|
0.01 ± 0.006
|
0.0492
|
↑
|
| |
Heba*14
|
0.02 ± 0.006
|
0.0127
|
↑
|
|
244
|
P*14
|
-0.008 ± 0.002
|
0.0016
|
↓
|
|
250
|
NB*2
|
0.007 ± 0.003
|
0.0417
|
↑
|
| |
NB*14
|
0.01 ± 0.005
|
0.0535
|
↑
|
|
251
|
P*2
|
0.04 ± 0.02
|
0.0449
|
↑
|
|
(β-casein precursor)
|
P*14
|
0.008 ± 0.002
|
0.0025
|
↑
|
|
341
|
P*14
|
0.02 ± 0.004
|
0.0006
|
↑
|
|
343
|
P*14
|
0.02 ± 0.009
|
0.0188
|
↑
|
|
346
|
NB*2
|
0.02 ± 0.01
|
0.0331
|
↑
|
|
349 (CPP3)
|
NB*2
|
0.01 ± 0.004
|
0.0084
|
↑
|
|
350
|
NB*2
|
0.02 ± 0.01
|
0.0423
|
↑
|
|
351
|
P*2
|
0.04 ± 0.01
|
0.0079
|
↑
|
| |
P*14
|
0.02 ± 0.01
|
0.0467
|
↑
|
|
352
|
P*2
|
0.01 ± 0.006
|
0.0411
|
↑
|
|
354
|
NB*2
|
-0.02 ± 0.004
|
< 0.0001
|
↓
|
|
(β-Lactoglobulin)
|
NB*14
|
-0.02 ± 0.007
|
0.0105
|
↓
|
| |
Heba*14
|
0.01 ± 0.007
|
0.0459
|
↑
|
|
355
|
NB*2
|
0.01 ± 0.005
|
0.0481
|
↑
|
|
358
|
NB*2
|
0.05 ± 0.02
|
0.0216
|
↑
|
|
359
|
NB*2
|
0.01 ± 0.005
|
0.0494
|
↑
|
| |
P*2
|
0.01 ± 0.005
|
0.0474
|
↑
|
- NB, Newbould 305; P, Parent 3231; Heba, SCV Heba 3231
- Trmt*Day denotes the treatment and day of the observed change in protein spot
- CPP3, component 3 of the proteose peptone
- Data are presented as the least square mean ± standard error of the mean (LSM ± SEM).
- Direction indicates the up-or down-regulation of the protein spot on day 2 or 14 pi, compared to baseline.
- Spot I.D. number refers to protein spot number assigned by the Melanie software. Spots that were excised and identified by LC-MS/MS are indicated in parentheses.
