Changes in Holstein cow milk and serum proteins during intramammary infection with three different strains of Staphylococcus aureus

Table 1 Change in milk protein expression on 2 and 14 days post-intramammary infection with different S.aureus strains

Spot I.D.	Trmt*Day	LSM ± SEM	p-vlaue	Direction
11	P*14	0.03 ± 0.01	0.0205	↑
	Heba*14	0.02 ± 0.01	0.0382	↑
12	P*2	0.05 ± 0.02	0.0127	↑
13	P*2	0.02 ± 0.01	0.0206	↑
	Heba*14	0.02 ± 0.01	0.024	↑
21	P*14	0.03 ± 0.01	0.0397	↑
33	P*2	0.05 ± 0.02	0.0056	↑
35	NB*2	0.02 ± 0.004	0.0041	↑
	Heba*14	0.01 ± 0.005	0.0467	↑
39 (CPP3)	P*2	0.02 ± 0.007	0.0476	↑
	P*14	0.01 ± 0.004	0.0032	↑
	Heba*14	0.01 ± 0.004	0.0154	↑
45	NB*2	0.01 ± 0.004	0.0122	↑
131	NB*2	0.01 ± 0.005	0.031	↑
141	NB*2	0.04 ± 0.01	0.0296	↑
212	P*2	0.01 ± 0.004	0.0038	↑
	Heba*14	0.01 ± 0.005	0.0408	↑
220	P*2	0.04 ± 0.01	0.0214	↑
	Heba*14	0.01 ± 0.005	0.0304	↑
222	NB*2	0.02 ± 0.009	0.0384	↑
227	P*2	0.008 ± 0.003	0.0127	↑
235 (CPP3)	P*2	0.03 ± 0.01	0.0241	↑
	P*14	0.01 ± 0.006	0.0492	↑
	Heba*14	0.02 ± 0.006	0.0127	↑
244	P*14	-0.008 ± 0.002	0.0016	↓
250	NB*2	0.007 ± 0.003	0.0417	↑
	NB*14	0.01 ± 0.005	0.0535	↑
251	P*2	0.04 ± 0.02	0.0449	↑
(β-casein precursor)	P*14	0.008 ± 0.002	0.0025	↑
341	P*14	0.02 ± 0.004	0.0006	↑
343	P*14	0.02 ± 0.009	0.0188	↑
346	NB*2	0.02 ± 0.01	0.0331	↑
349 (CPP3)	NB*2	0.01 ± 0.004	0.0084	↑
350	NB*2	0.02 ± 0.01	0.0423	↑
351	P*2	0.04 ± 0.01	0.0079	↑
	P*14	0.02 ± 0.01	0.0467	↑
352	P*2	0.01 ± 0.006	0.0411	↑
354	NB*2	-0.02 ± 0.004	< 0.0001	↓
(β-Lactoglobulin)	NB*14	-0.02 ± 0.007	0.0105	↓
	Heba*14	0.01 ± 0.007	0.0459	↑
355	NB*2	0.01 ± 0.005	0.0481	↑
358	NB*2	0.05 ± 0.02	0.0216	↑
359	NB*2	0.01 ± 0.005	0.0494	↑
	P*2	0.01 ± 0.005	0.0474	↑

NB, Newbould 305; P, Parent 3231; Heba, SCV Heba 3231
Trmt*Day denotes the treatment and day of the observed change in protein spot
CPP3, component 3 of the proteose peptone
Data are presented as the least square mean ± standard error of the mean (LSM ± SEM).
Direction indicates the up-or down-regulation of the protein spot on day 2 or 14 pi, compared to baseline.
Spot I.D. number refers to protein spot number assigned by the Melanie software. Spots that were excised and identified by LC-MS/MS are indicated in parentheses.

ISSN: 1746-6148