Two-dimensional gel electrophoresis of milk proteins. Proteins were resolved on a 13 cm Immobiline DryStrip, pH 3-10, followed by 12.5% SDS-PAGE. Circled spots are significantly different in protein expression between treatment groups as well as on day 2 or 14 pi, relative to day 0 values. Corresponding graphs detail the least square mean of the log transformed spot intensities ± SEM for each spot. Significant changes in protein expression levels relative to baseline (day 0) are denoted by asterisks (P < 0.05* and P < 0.01**). a,b significant differences in protein expression between treatment groups on day 2 or 14 pi with raw p-values and FDR-adjusted p-values presented (P < 0.05).