BMC Veterinary Research

Table 2 Results of increasing starting DNA on real-time PCR assay performance.

From: Improved specificity for detection of Mycobacterium bovis in fresh tissues using IS6110 real-time PCR

	Sample
	01916^a		4022^a		4477^b		01870^a		04034^a
DNA^c	IS6110_T	β-actin	IS6110_T	β-actin	IS6110_T	β-actin	IS6110_T	β-actin	IS6110_T	β-actin
100	+	+	-	+	-	+	-	+	-	+
250	+	+	+	+	-	+	-	+	-	+
500	+	+	+	+	-	+	-	+	-	+
1000	+	+	+	+	-	+	-	+	-	+

^a M. bovis infected tissues
^b M. smegmatis infected tissues
^c DNA isolated from homogenized tissues added to PCR reaction in nanograms.

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ISSN: 1746-6148

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