Skip to main content

Advertisement

Table 2 Results of increasing starting DNA on real-time PCR assay performance.

From: Improved specificity for detection of Mycobacterium bovis in fresh tissues using IS6110 real-time PCR

  Sample
  01916a 4022a 4477b 01870a 04034a
DNAc IS6110_T β-actin IS6110_T β-actin IS6110_T β-actin IS6110_T β-actin IS6110_T β-actin
100 + + - + - + - + - +
250 + + + + - + - + - +
500 + + + + - + - + - +
1000 + + + + - + - + - +
  1. a M. bovis infected tissues
  2. b M. smegmatis infected tissues
  3. c DNA isolated from homogenized tissues added to PCR reaction in nanograms.