Multiple-locus, variable number of tandem repeat analysis (MLVA) of the fish-pathogen Francisella noatunensis

BMC Veterinary Research

Table 6 Location of Blastn matches from Variable Number of Tandem Repeat -sequences from F. philomiragi a DSM7535^T.

Marker locus	BP in query	Identity	Location	Adjacent genes	E-value
Fnn-VNTR2	186	96%	Intergenic (1900473-1900533)	Bor lipoprotein/NADPH-quinone reductase	7 × 10^-28
Fnn-VNTR4	394	97%	Intragenic (695610- 695415)	DNA-directed DNA polymerase	1 × 10^-88
Fnn-VNTR5 ^A	333	99%	Intragenic (495433-496083)	FTN_0396 hypothetical protein	3 × 10^-79
Fnn-VNTR6	327	100%	Intragenic (1129799-1130125)	50S ribosomal protein L10	7 × 10^-170
Fnn-VNTR7	458	97%	Intragenic (1661303-1660846)	FTN_1059 hypothetical and trigger factor protein	0

The Blastn search was performed using the complete amplicon of the primer pairs. All locations were identified in F. philomiragia ATCC25017^T (Accession number CP000937). Sequences were derived from F. philomiragia DSM7535^T unless otherwise stated.
VNTR-sequence obtained from F. noatunensis ssp. noatunensis GM2212^A

ISSN: 1746-6148