Multiple-locus, variable number of tandem repeat analysis (MLVA) of the fish-pathogen Francisella noatunensis

BMC Veterinary Research

Table 4 Allelic profiles from the stability testing.

Isolate	VNTR1	VNTR2	VNTR3	VNTR4	VNTR5	VNTR6	VNTR7
GM2212	211nt - 7r	217nt - 8r	212nt - 10r	311nt - 10r	312nt - 5r	301nt - 10er	435nt - 5r
GM2212-P10	219nt - 8r	217nt - 8r	212nt - 10r	311nt - 10r	312nt - 5r	301nt - 10er	435nt - 5r
Fc10	211nt - 7r	217nt - 8r	212nt - 10r	311nt - 10r	312nt - 5r	301nt - 10er	435nt - 5r
Fc14	211nt - 7r	217nt - 8r	212nt - 10r	311nt - 10r	312nt - 5r	301nt - 10er	435nt - 5r
Fc18	211nt - 7r	217nt - 8r	212nt - 10r	311nt - 10r	312nt - 5r	395nt - 9er	435nt - 5r

In vivo stability was shown on passage 10 (P10) of the GM2212 isolate and in vitro stability on infected cod held at 10 (Fc10), 14 (Fc14) and 18°C (Fc18). Results are displayed with number of nucleotides in repeat marker and numbers of repeats. Two shifts were observed in GM2212, VNTR-1 after 10 passages on CHAB agar at 20°C and in VNTR-6 after passage in cod held at 18°C. The original sequence is shown in bold, whereas the mutations are in italics.
Heterogeneous repeats with Single nucleotide polymorphism (snp), noted as nucleotide number in repeat sequence (nt) and at repeat of occurrence (r); e = 2 snp C-T nt1 r4 r6

ISSN: 1746-6148