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Table 4 Detection rates of Mycoplasma mycoides subsp. mycoides SC in CBPP-infected animals after parallel investigation of lung, lymph nodes and pleural fluid by cultivation, MSC_0136 and MSC_1046 rt-PCR and conventional SC3-PCR assay

From: Assessment of a novel multiplex real-time PCR assay for the detection of the CBPP agent Mycoplasma mycoides subsp. mycoidesSC through experimental infection in cattle

Animal: n = 19, Condition: positive = 19

  

rt-PCR

PCR

 

Culture

MSC_0136

MSC_1046

SC3

Positives

17

19

19

15

Detection rate

89.5%

100%

100%

78.9%

  1. n = total number, rt: real time.