Assessment of a novel multiplex real-time PCR assay for the detection of the CBPP agent Mycoplasma mycoides subsp. mycoidesSC through experimental infection in cattle

Table 2 Primers and TaqMan^® probes

Target	Primers/Probes	Sequence (5'-3')	Position²
IppQ MSC_0136 IAC	MSC_1046-S MSC_1046-R2 MSC_1046-TM1 MSC_0136-F MSC_0136-R MSC_0136-TM EGFP-1F EGFP-10R EGFP-Hex	ATCAAGATATTTCGAGTTGAAATGTAAG TGTATATTTTTTAGATTTCAATCTGAAAGTG FAM-TTTCAGCTCGATAAAACATATTT-BBQ¹ AACACAAAAAACCGAACAGCCT AGCTTATCAGGAACTTTTTTAACGTA Cy5-TGAGATAGTTCAAATTGGCTTT-BBQ¹ GACCACTACCAGCAGAACAC CTTGTACAGCTCGTCCATGC HEX-AGCACCCAGTCCGCCCTGAGCA-BHQ1	1166686-1166713 1190443-1190470 1166797-1166767 1190554-1190524 1166754-1166732 1190511-1190489 159994-160015 1023331-1023310 160131-160106 1023194-1023219 160042-160063 1023283-1023262

¹Locked nucleic acid (LNA) containing nucleotides are underlined,²Based on GenBank sequence BX293980

ISSN: 1746-6148