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Figure 1 | BMC Veterinary Research

Figure 1

From: Assessment of a novel multiplex real-time PCR assay for the detection of the CBPP agent Mycoplasma mycoides subsp. mycoidesSC through experimental infection in cattle

Figure 1

Real-time quantification of DNA extracted from Mmm SC cultures. DNA concentration was measured spectrophotometrically and adjusted to 100 ng μl-1. Five tenfold dilution series were prepared and applied in duplex rt-PCR assays comprising the amplification and detection of the targets MSC_0136 (A) and MSC_1046 (B) with the TaqMan® LNA probes FAM-MSC_1046 and Cy5-MSC_0136, respectively. The detection limits are 10 fg DNA and 100 fg DNA corresponding to approximately 8 genome equivalents and 80 genome equivalents for the MSC_0136 and MSC_1046 assay, respectively.

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