Figure 4

Representative photomicrographs showing testicular histology following 60 days after single bilateral intra-testicular injection of 0.25 ml 10% sterile analytical grade of calcium chloride solution containing 1% lignocaine hydrochloride in mature male cats showed coagulative necrosis in the seminiferous epithelium and the interstitial spaces as well as presence of degenerated and coagulated germ cells in combination with fibrous tissue and infiltration of leucocytes in tubular and interstitial spaces (E & F) or 20% sterile analytical grade of calcium chloride solution containing 1% lignocaine hydrochloride in mature male cats resulted complete testicular necrosis along with only presence of fibrous tissue and hyaline tissue and neither presence of germ cells or Leydig cells (G & H).