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Table 1 Primer sequences and UPL probes used for qRT-PCR amplification of selected target and reference genes.

From: Matrix metalloproteinases and their inhibitors in canine mammary tumors

Genes Accession number Primer sequence (5'-3') UPL probe
MMP-2 [GenBank:XM_535300.2] F: gggaccacggaagactatga
R: atagtggacatggcggtctc
29
MMP-9 [GenBank:NM_001003219.1] F: tgagaactaatctcactgacaagca
R: gctcggccacttgagtgta
6
MMP-13 [GenBank:XM_536598.2] F: ctcttcttctcgggaaacca
R: gcctggggtagtctttatcca
50
MT1-MMP [GenBank:XM_843664.1] F: gatctgaatgggaatgacatctt
R: gatggccgagggatcatt
76
TIMP-1 [GenBank:NM_001003182.1] F: cagggcctgtacctgtgc
R: cctgatgacgatttgggagt
112
TIMP-2 [GenBank:NM_001003082.1] F: atgagatcaagcagataaagatgttc
R: ggaggaaggagccgtgtag
93
TIMP-3 [GenBank:XM_538410.2] F: tgctgacaggccgcgt
R: gcagttacagcccaggtga
14
RECK [GenBank:NM_001002985.1] F: aaggggtgtctgtctggagat
R: cccaatttgcaaccttgaac
97
CGI-119 [GenBank:XM_531662.2] F: tctacaatctaagagagatttcagcaa
R: ttcctgacaagcacaaaatcc
15
GOLGA-1 [GenBank:XM_537849.2] F: ggtggctcaggaagttcaga
R: tatacggctgctctcctggt
149
  1. F: forward; R: reverse