Table 1 Primer sequences and UPL probes used for qRT-PCR amplification of selected target and reference genes.
From: Matrix metalloproteinases and their inhibitors in canine mammary tumors
Genes | Accession number | Primer sequence (5'-3') | UPL probe |
|---|---|---|---|
MMP-2 | [GenBank:XM_535300.2] | F: gggaccacggaagactatga R: atagtggacatggcggtctc | 29 |
MMP-9 | [GenBank:NM_001003219.1] | F: tgagaactaatctcactgacaagca R: gctcggccacttgagtgta | 6 |
MMP-13 | [GenBank:XM_536598.2] | F: ctcttcttctcgggaaacca R: gcctggggtagtctttatcca | 50 |
MT1-MMP | [GenBank:XM_843664.1] | F: gatctgaatgggaatgacatctt R: gatggccgagggatcatt | 76 |
TIMP-1 | [GenBank:NM_001003182.1] | F: cagggcctgtacctgtgc R: cctgatgacgatttgggagt | 112 |
TIMP-2 | [GenBank:NM_001003082.1] | F: atgagatcaagcagataaagatgttc R: ggaggaaggagccgtgtag | 93 |
TIMP-3 | [GenBank:XM_538410.2] | F: tgctgacaggccgcgt R: gcagttacagcccaggtga | 14 |
RECK | [GenBank:NM_001002985.1] | F: aaggggtgtctgtctggagat R: cccaatttgcaaccttgaac | 97 |
CGI-119 | [GenBank:XM_531662.2] | F: tctacaatctaagagagatttcagcaa R: ttcctgacaagcacaaaatcc | 15 |
GOLGA-1 | [GenBank:XM_537849.2] | F: ggtggctcaggaagttcaga R: tatacggctgctctcctggt | 149 |