Figure 4
TEER measurements and tight junction detection in P 0 and P 1 EBEC cultures at ALI. [a-b] Time-course of TEER development in P0 (a) and P1 (b) insert cultures; the abscissa indicates culture days before (negative) and after (positive) ALI creation (day 0); TEER values (Ω·cm2) at each time-point are expressed as mean ± SEM of n = 5 (P0) or n = 6 (P1); insets show TEER graphs of individual subjects. [c-d] Representative images of time-dependent immunofluorescence staining of P0 (c) and P1 (d) insert cultures for the tight junction protein ZO-1 (one black and two white squares: ALI day 0, immediately before ALI creation; two black and one white squares: intermediate time-point of the ALI-culture phase leading to bioelectrical stabilization; all black squares: mature and electrically stable ALI-cultures) (objective magnification: ×40). Nuclei are blue stained with DAPI.