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Figure 3 | BMC Veterinary Research

Figure 3

From: Growth and differentiation of primary and passaged equine bronchial epithelial cells under conventional and air-liquid-interface culture conditions

Figure 3

Features of mucociliary differentiation of EBEC cultured on solid supports in serum-free or serum-containing medium. [a] PAS-positive (magenta coloured) cells in 30 days old EBEC cultures on glass cover slips in serum-containing medium (objective magnification: ×10); inset shows less intense PAS-positive stain in EBEC grown in serum-free medium (objective magnification: ×40). [b] Ciliated cells detected by phase contrast microscopy in 30 days old EBEC cultures on tissue culture flasks and glass cover slips in serum-containing medium; arrows indicate cilia (objective magnification: ×40).

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