Figure 6

In situ hybridization with natural MSGS specimen. Example of an in situ hybridization reaction using a rhodamine-labeled ICE probe (red) together with an FITC-labeled LSNV probe (green) with lymphoid organ (LO) tissue of a P. monodon specimen from an MSGS pond that was positive by RT-PCR for both ICE and LSNV. a) Phase contrast image of tissue; b) Image of LSNV fluorescence; c) Image of ICE fluorescence; d) Combined images of a to c with a magnified insert showing that the fluorescence distribution for the two signals is single (green or red) and combined (yellow).