ICE sequence. Putative open reading frame of 2233 nucleotides in ICE (GenBank accession number FJ498866) corresponding to an uninterrupted sequence of 744 deduced amino acids by +1 frame translation. A putative phage integrase domain is outlined in dark grey in the deduced amino acid sequence. The three probe sequences (Probe 1 of 397 bp; Probe 2 of 642 bp; Probe 3 of 399 bp) are outlined sequentially in light grey with their primer sequences underlined. A single fragment of 1958 bp could also be amplified using the forward primer of Probe 1 and the reverse primer of Probe 3.