Table 3 Sequence, concentration and position of specific oligonucleotide probes for B. burgdorferi sensu lato
Type of primers and isolate | Oligonucleotide Sequence(5'-3') | Concentration used (pmol) | Position on 5S-23S intergenic spacer region |
|---|---|---|---|
23SN1 | ACCATAGACTCTTATTACTTTGAC | 50 | 469-446 |
23SC | TAAGCTGACTAATACTAATTACCC | 50 | 92-115 |
5SCB | biotin-GAGAGTAGGTTATTGCCAGGG | 50 | 243-263 |
23SN2 | ACCATAGACTCTTATTACTTTGACCA | 50 | 469-444 |
B.burgdorfei sensu lato (S1) | a-CTTTGACCATATTTTTATCTTCCA | 800 | 453-430 |
B. burgdorferi sensu stricto (Ss) | a-AACACCAATATTTAAAAAACATAA | 20 | 322-299 |
B. garinii (Ga) | a-AACATGAACATCTAAAAACATAAA | 10 | 322-298 |
B. afzelii (Af) | a-AACATTTAAAAAATAAATTCAAGG | 200 | 305-278 |
B. valaisiana (Vs) | a-CATTAAAAAAATATAAAAAATAAATTTAAGG | 10 | 303-278 |