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Table 1 Results of MASS-SPEC analysis of 9 spots excised from 2DE gel of SF-PPD

From: Antibody response to a sterile filtered PPD tuberculin in M. bovis infected and M. bovis sensitized cattle

SF-PPD spots for MASS SPEC analysis

Mascot Search Results

 

~ 2DE spot location

Protein hits

Designation

mass(kDa)

pl

Name

mass(kDa)

Score

MPB83

22

4.2

MPB83

22.2

471

   

CFP10

10.8

458

   

MPB70

22.5

433

   

MPB63

13.7

430

   

ESAT-6

10.0

337

MPB70

22

4.7

MPB70

22.5

588

   

MPB83

22.2

473

   

Rv1314c

20.8

196

   

CFP10

10.8

177

   

MPB63

16.5

47

MPB64

25

4.5

MPB64

25.1

733

   

MPB83

22.2

509

   

MPB70

22.5

434

   

M.tb protease

23.5

381

   

lipoprotein LPPX

24.3

377

MPB83 & MPB64

25

5.4

Adenylate kinase

20.1

504

   

MPB83

22.2

466

   

MPB64

25.1

366

   

Rv2557

24.7

356

   

Ag85b

30.8

339

MPB51

27

6

MPB51

31.1

699

   

enoyl-CoA hydratase

24.5

506

   

MPB64

25.1

145

   

MPB70

22.5

122

   

Peptide of a 24 kDa immunoprotective protein

2.1

99

Ag85b

31

5

Ag85b

30.8

609

   

M.tb protein MT3693

28.2

534

   

Beta-1, glucanase precursor

32.2

329

   

chaperonin GroEL

31.2

289

   

29 kDa Ag

28.5

210

Ag85b & Ag85c

31

5.3

Ag85b

30.8

610

   

Ag85c

31.2

417

   

Ag85a

32.7

276

   

Conserved membrane protein

27.3

107

   

dehydrogenase/reductase

29.9

42

Ag85c

32

5.3

Ag85c

33.1

632

   

esterase

34.0

611

   

Ag85b

30.8

289

   

Ag85a

32.8

191

Ag85c & Ag85a

32

5.7

Ag85c

33.1

578

   

Ag85a

32.8

351

   

Ag85b

30.8

309

   

amidohydrolase

29.1

181

   

TB15.3

15.3

133

  1. Protein spots were excised from a CBB stained 2-DE gel of SF-PPD (200 μg; 50 μL of 4 μg/μL) and submitted to the Ottawa Institute of Systems Biology, University of Ottawa for MASS-SPEC analysis. In the table, the five most probable candidate proteins as determined by Mascot search results are listed for each submitted spot. Bold text is used to identify the most likely constituent or majority constituent protein for each submitted spot. Presumptive identifications are based on data provided by the Mascot search results and previously performed 2DE analysis of Mycobacterium spp. CFPs [15, 20, 27, 28, 32, 35, 40].