|
SF-PPD spots for MASS SPEC analysis
|
Mascot Search Results
|
|---|
| |
~ 2DE spot location
|
Protein hits
|
|---|
|
Designation
|
mass(kDa)
|
pl
|
Name
|
mass(kDa)
|
Score
|
|
MPB83
|
22
|
4.2
|
MPB83
|
22.2
|
471
|
| | | |
CFP10
|
10.8
|
458
|
| | | |
MPB70
|
22.5
|
433
|
| | | |
MPB63
|
13.7
|
430
|
| | | |
ESAT-6
|
10.0
|
337
|
|
MPB70
|
22
|
4.7
|
MPB70
|
22.5
|
588
|
| | | |
MPB83
|
22.2
|
473
|
| | | |
Rv1314c
|
20.8
|
196
|
| | | |
CFP10
|
10.8
|
177
|
| | | |
MPB63
|
16.5
|
47
|
|
MPB64
|
25
|
4.5
|
MPB64
|
25.1
|
733
|
| | | |
MPB83
|
22.2
|
509
|
| | | |
MPB70
|
22.5
|
434
|
| | | |
M.tb protease
|
23.5
|
381
|
| | | |
lipoprotein LPPX
|
24.3
|
377
|
|
MPB83 & MPB64
|
25
|
5.4
|
Adenylate kinase
|
20.1
|
504
|
| | | |
MPB83
|
22.2
|
466
|
| | | |
MPB64
|
25.1
|
366
|
| | | |
Rv2557
|
24.7
|
356
|
| | | |
Ag85b
|
30.8
|
339
|
|
MPB51
|
27
|
6
|
MPB51
|
31.1
|
699
|
| | | |
enoyl-CoA hydratase
|
24.5
|
506
|
| | | |
MPB64
|
25.1
|
145
|
| | | |
MPB70
|
22.5
|
122
|
| | | |
Peptide of a 24 kDa immunoprotective protein
|
2.1
|
99
|
|
Ag85b
|
31
|
5
|
Ag85b
|
30.8
|
609
|
| | | |
M.tb protein MT3693
|
28.2
|
534
|
| | | |
Beta-1, glucanase precursor
|
32.2
|
329
|
| | | |
chaperonin GroEL
|
31.2
|
289
|
| | | |
29 kDa Ag
|
28.5
|
210
|
|
Ag85b & Ag85c
|
31
|
5.3
|
Ag85b
|
30.8
|
610
|
| | | |
Ag85c
|
31.2
|
417
|
| | | |
Ag85a
|
32.7
|
276
|
| | | |
Conserved membrane protein
|
27.3
|
107
|
| | | |
dehydrogenase/reductase
|
29.9
|
42
|
|
Ag85c
|
32
|
5.3
|
Ag85c
|
33.1
|
632
|
| | | |
esterase
|
34.0
|
611
|
| | | |
Ag85b
|
30.8
|
289
|
| | | |
Ag85a
|
32.8
|
191
|
|
Ag85c & Ag85a
|
32
|
5.7
|
Ag85c
|
33.1
|
578
|
| | | |
Ag85a
|
32.8
|
351
|
| | | |
Ag85b
|
30.8
|
309
|
| | | |
amidohydrolase
|
29.1
|
181
|
| | | |
TB15.3
|
15.3
|
133
|
- Protein spots were excised from a CBB stained 2-DE gel of SF-PPD (200 μg; 50 μL of 4 μg/μL) and submitted to the Ottawa Institute of Systems Biology, University of Ottawa for MASS-SPEC analysis. In the table, the five most probable candidate proteins as determined by Mascot search results are listed for each submitted spot. Bold text is used to identify the most likely constituent or majority constituent protein for each submitted spot. Presumptive identifications are based on data provided by the Mascot search results and previously performed 2DE analysis of Mycobacterium spp. CFPs [15, 20, 27, 28, 32, 35, 40].
