Table 1 Primary antibodies used for immunohistochemistry in the current study.
From: Molecular-based tumour subtypes of canine mammary carcinomas assessed by immunohistochemistry
Antibody (anti-) | Clone | Manufacturer | Working concentration | |
|---|---|---|---|---|
Oestrogen Receptor | polyclonal | Â | Zymed (South San Francisco, Ca) | 1:50 |
Progesterone Receptor | monoclonal | PR 4-12 | EMD Biosciences (San Diego, Ca) | 1:10 |
ERB-B2 | polyclonal | Â | Dako (Glostrup, Denmark) | 1:50 |
Cytokeratin 14 | monoclonal | Ab-1 (LL002) | NeoMarkers (Fremont, Ca) | 1:300 |
Cytokeratins 5/6 | monoclonal | D5/16B4 | Zymed (South San Francisco, Ca) | 1:100 |