Overview of the GSEA method applied. (A) Heat map of the gene set 'DHEA_IM_vs_CTR3' containing all genes found significantly up-regulated (> 1.5 fold and p-value <0.05) when comparing DHEA IM treated animals versus the control population of experiment 3. Colours range from dark red to dark blue representing respectively the highest and lowest expression of a gene. (B) This gene set was compared to the ranked list of the total microarray expression data set of DHEA IM treated and CTR1 animals showing a significant (p-value 0.000 and FDR of 0.000) enrichment of genes in DHEA IM treated animals when compared to the control group of experiment 1. (C) Heat map displaying the genes of gene set 'DHEA_IM_vs_CTR3' in DHEAIM and CTR1 animals.