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Figure 6 | BMC Veterinary Research

Figure 6

From: Vaccination with attenuated Salmonella enterica Dublin expressing E coli O157:H7 outer membrane protein Intimin induces transient reduction of fecal shedding of E coliO157:H7 in cattle

Figure 6

Intimin specific IgA secreting cells in peripheral blood mononuclear cells. 105 PBMC were plated on bovine IgA-coated wells. Cells were stimulated with intimin (200 ng per well) and incubated for 18 hrs at 37°C. After incubation, cells were incubated with anti-IgA antibody conjugated to HRPO, and spots were developed using substrate solution. Intimin-specific IgA-secreting cells were determined by subtracting the number of spots obtained in wells without any stimulant from the number of spots obtained in wells to which intimin was added. The bar graph depicts the mean (± standard deviation) of animals in each group.

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BMC Veterinary Research

ISSN: 1746-6148

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