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Figure 2 | BMC Veterinary Research

Figure 2

From: Vaccination with attenuated Salmonella enterica Dublin expressing E coli O157:H7 outer membrane protein Intimin induces transient reduction of fecal shedding of E coliO157:H7 in cattle

Figure 2

Time frame of experiment and collection of samples. Calves were divided into 3 treatment groups. Calves (n = 3) in the control group were fed only milk on days 0, 14 and 28. Calves (n = 4) in the vector group were orally inoculated with the live attenuated non-recombinant S. Dublin strain on days 0, 14 and 28. Calves (n = 5) in the vaccinated group were inoculated orally with live attenuated recombinant S. Dublin expressing E. coli O157:H7 intimin on days 0, 14 and 28. All calves were challenged orally with E. coli O157:H7 on day 98 post-vaccination. Fecal samples from calves were collected daily from all calves from day 0 to day 42 post-inoculation for Salmonella culture, and fecal samples were collected weekly thereafter. After oral E. coli O157:H7 challenge, fecal samples were collected daily to monitor E. coli O157:H7 shedding. Peripheral blood samples were collected weekly after inoculation to measure levels of serum IgA and to detect intimin-specific IgA-secreting cells by ELISpot assay.

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