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Figure 1 | BMC Veterinary Research

Figure 1

From: Vaccination with attenuated Salmonella enterica Dublin expressing E coli O157:H7 outer membrane protein Intimin induces transient reduction of fecal shedding of E coliO157:H7 in cattle

Figure 1

Expression of Intimin in aroA mutant S . Dublin. Protein from overnight cultures of S. Dublin aroA, S. Dublin aroA with pRB3::eaeA, or E. coli K12 with pEB310 were separated on 4-20% SDS-PAGE and expression of intimin determined by western blot. Lane 1, S. Dublin aroA, lane 2 S. Dublin aroA with pRB3::eaeA, and lane 3, E. coli K12 with pEB310. The full-length intimin protein is 96 kD (upper arrow) present in E. coli K12 with pEB310 but absent in S. Dublin aroA with pRB3::eaeA. A smaller immunoreactive protein (lower arrow) is present in both S. Dublin with pRB3::eaeA and E. coli K12 with pEB310.

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BMC Veterinary Research

ISSN: 1746-6148

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