Detection of squirrel poxvirus by nested and real-time PCR from red (Sciurus vulgaris) and grey (Sciurus carolinensis) squirrels

Table 2 Primers used in the nested and real-time squirrelpox virus PCRs.

Stage	Primer orientation	Sequence (5' - 3')
1^st round nested PCR	SqP1 - forward SqP2 - reverse	GCGGCCGCGCTGACCGCCATCG CCAGCACGACTTCTTCCTGGAG
2^nd round nested PCR	SqP3 - forward SqP4 - reverse	CGCTCGCGTGTCCTACAGCCTG GCAGTCCGCAGCGCGCGCAGAT
Real-time PCR primers	SP7 - forward SP8 - reverse	GGGCGATCGTGCCGCTCAG CGCCTCGAGGTCCGGCTC

ISSN: 1746-6148