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Table 1 Comparison of nested and real-time PCR results from 39 grey skin samples

From: Detection of squirrel poxvirus by nested and real-time PCR from red (Sciurus vulgaris) and grey (Sciurus carolinensis) squirrels

  Nested PCR results
  Positive Negative
Real-time PCR results Positivea 13 3
  Negativeb 4 15
  Equivocalc 1 3
  1. a 3/3 or 2/3 of the triplicates positive Ct and appropriate melt curve.
  2. b 0/3 positive Ct and no melt curve.
  3. c 1/3 positive Ct and melt curve.